Protein Heterogeneity in Rat CNS Myelin Subfractions
- 1 January 1978
- book chapter
- Published by Springer Nature
- Vol. 100, 117-133
- https://doi.org/10.1007/978-1-4684-2514-7_8
Abstract
Microsomal fraction-free myelin from forebrain and spinal cord of young and mature rats, when subjected to hypo-osmotic shock and slow speed centrifugation, yielded a myelin pellet and a supernatant fraction (SN 4). Fraction SN 4 consisted of small vesicular profiles in which the major myelin proteins were reduced whereas high molecular weight material such as Wolfgram protein, myelin-associated glycoprotein and CNP were substantially increased over myelin. A close correlation of the SN 4 fraction to the myelin-like fraction of Davison and coworkers was suggested. The myelin pellets were subfractioned on zonal sucrose gradients to yield bell-shaped particle distributions. Besides shifts in densities of the maxima between myelin of young and mature forebrain and spinal cord, a decrease was observed from the light to the heavy gradient end in basic proteins, and an increase in Wolfgram protein and other high molecular weight proteins. Proteolipid protein took an intermediate position. Light fractions from adult spinal cord displayed CNP activities below those of the total homogenate. This result, together with the very high CNP activities in fraction SN 4 casts some doubt on CNP being a marker for compact myelin; rather it appears that CNP is a marker for the process of myelin formation.This publication has 31 references indexed in Scilit:
- SOLUBILIZATION AND CHARACTERIZATION OF THE RAT BRAIN CHOLESTEROL ESTER HYDROLASE LOCALIZED IN THE MYELIN SHEATHJournal of Neurochemistry, 1977
- METABOLIC RELATIONSHIPS BETWEEN MYELIN SUBFRACTIONS: ENTRY OF PROTEINS 1Journal of Neurochemistry, 1976
- METABOLIC RELATIONSHIPS BETWEEN MYELIN SUBFRACTIONS: ENTRY OF GALACTOLIPIDS AND PHOSPHOLIPIDS 1Journal of Neurochemistry, 1976
- SIMULTANEOUS ISOLATION OF PURIFIED MICROSOMAL and MYELIN FRACTIONS FROM RAT SPINAL CORDJournal of Neurochemistry, 1976
- Morphological and biochemical characterization of light and heavy myelin isolated from developing rat brainBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- CHARACTERIZATION AND PROTEIN ANALYSIS OF MYELIN SUBFRACTIONS IN RAT BRAIN: DEVELOPMENTAL AND REGIONAL COMPARISONSJournal of Neurochemistry, 1975
- STUDIES OF THE MECHANISM OF DEMYELINATION REGIONAL DIFFERENCES IN MYELIN STABILITY IN VITROJournal of Neurochemistry, 1975
- MEMBRANE PROTEINS OF RAT BRAIN: COMPOSITIONAL CHANGES DURING POSTNATAL DEVELOPMENTJournal of Neurochemistry, 1974
- MYELIN SUBFRACTIONS IN DEVELOPING RAT BRAIN: CHARACTERIZATION AND SULPHATIDE METABOLISMJournal of Neurochemistry, 1973
- Proteins of rat brain myelin. Extraction with sodium dodecylsulphate and electrophoresis on analytical and preparative scaleFEBS Letters, 1970