Management of Pyridoxine and Pyridoxal in the Isolated Kidney of the Rat
- 1 August 1980
- journal article
- research article
- Published by Elsevier in Journal of Nutrition
- Vol. 110 (8), 1597-1609
- https://doi.org/10.1093/jn/110.8.1597
Abstract
The vascularly perfused kidney of the rat was used to study the transport, metabolism, filtration, secretion, reabsorption and excretion of pyridoxine (PN) and pyridoxal (PL), independent of other tissues including erythrocytes. [3H]-PN or [3H]-PL was administered via the perfusate at concentrations ranging from 8 nmole to 20.75 µmole per 95 ml perfusate. After 30 minutes of perfusion with 8 nmole of [3H]-PN, 17.2% of the total 3H was found in the kidney. As the concentration of PN in the perfusate was increased the percentage found in the kidney decreased. This inverse relationship resulted primarily from saturation of PL kinase in the kidney. At all levels of administration approximately one-half as much 3H was found in the kidney after [3H]-PL administration as when [3H]-PN was given at the same concentration. In the case of PN the primary metabolite found in the kidney was PNP whereas when PL was administered, significant amounts of PLP, PMP and pyridoxic acid were found. A differential response in the renal tubular handling of PN and PL is also reported. At all levels of administration of PL, the majority of the vitamer filtered was reabsorbed. The same was the case at the lowest dosage, however, at higher concentrations there was a net secretion of PN into the urine via a saturable process, this could account for the observed ratio of greater than 1 of kidney/perfusate concentration of PN.Keywords
This publication has 28 references indexed in Scilit:
- Transport and metabolism of pyridoxine in rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1980
- VITAMIN B6 TRANSPORT IN THE CENTRAL NERVOUS SYSTEM: IN VIVO STUDIESJournal of Neurochemistry, 1978
- VITAMIN B6 TRANSPORT IN THE CENTRAL NERVOUS SYSTEM: IN VITRO STUDIESJournal of Neurochemistry, 1978
- IMPROVED CHROMATOGRAPHIC SEPARATION AND FLUOROMETRIC DETERMINATION OF VITAMIN B6COMPOUNDS IN FOODSJournal of Food Science, 1977
- Regulation of pyridoxal 5′-phosphate metabolism in liverBiochemical and Biophysical Research Communications, 1974
- METABOLISM OF TRITIUM‐LABELLED PYRIDOXINE AND PYRIDOXINE 5′‐PHOSPHATE IN THE CENTRAL NERVOUS SYSTEM1Journal of Neurochemistry, 1973
- Metabolism of [14C]pyridoxol in the pregnant ratBiochimica et Biophysica Acta (BBA) - General Subjects, 1971
- The absorption of tritium-labelled pyridoxine HCl in control subjects and in patients with intestinal malabsorptionGut, 1964
- Transamination of Pyridoxamine and Amino Acids with Glyoxylic Acid1Journal of the American Chemical Society, 1954
- Preparation of Crystalline Phosphorylated Derivatives of Vitamin B6Journal of the American Chemical Society, 1954