Identification of 3β, 7α-Dihydroxychol-5-enoic Acid in Fistula Bile of the Rat Given Cholesterol-4-14C and DL-Mevalonate-2-14C

Abstract

A Lifschütz positive compound was found in the bile of bile-fistula rats injected with mevalonate-¹⁴C or with cholesterol-¹⁴C. This compound was identified as 3β, 7α-dihydroxychol-5-enoic acid by thin-layer chromatography and radio-isotope dilution experiments. This finding was taken for a further evidence favorable for a new pathway of chenodeoxycholic acid biogenesis proposed by the authors.