'METACHRONOUS' CLEAVAGE AND INITIATION OF GASTRULATION IN AMPHIBIAN EMBRYOS

Abstract

The cleavage pattern in the egg of Xenopus laevis was investigated with the aid of time-lapse cinematography. From the 5th cleavage onward, divisions of the surface blastomeres are not synchronous but metachronous. A few blastomeres in a very restricted region which is situated in most cases in the dorsal side of the animal hemisphere, slightly distant from the median line and near the equatorial junction of the animal and vegetal hemispheres, divide before the other blastomeres and a wave-like propagation of the divisions travels along the surface from that region toward the animal and vegetal poles. The wave-like propagation ends in the vegetal pole region. In the animal hemisphere this pattern of cleavage is continued until the 13th cleavage and thereafter the divisions of surface blastomers become asynchronous. In the vegetal pole region the 14th metachronous division of blastomeres is clearly observed in the film. Gastrulation begins after 14 cleavages.