Radioimmunoassay of human sex hormone binding globulin: improved radioiodination procedure
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 45 (5), 461-469
- https://doi.org/10.3109/00365518509155244
Abstract
Sex hormone binding globulin (SHBG), purified by affinity chromatography from retroplacental blood plasma, was reacted with 3-(p-hydroxyphenyl) propionic acid N-hydroxysuccinimidyl ester (PHPPS, Bolton-Hunter reagent). The derivative of SHBG obtained (parahydroxyphenylpropionyl-SHBG; PHPP-SHBG) was stable and could, in contrast to underived SHBG, be efficiently 125I-iodinated with a lactoperoxidase technique. The PHPP-SHBG labelled with 125I had good antiserum binding and stability properties and was used for radioimmunoassay (RIA) of SHBG in serum. The RIA requires a total incubation time of 3 h. It has been standardized with purified SHBG and has a sensitivity of 5 µg/1, giving a lowest detectable concentration in the routine procedure (samples diluted 1:40) of about 0.2 mg/1. Variation within and between assay was 4.1% and 7.2%, respectively, for samples with values within the normal range. Values obtained by this RIA procedure correlate well with those obtained by a dihydrotestosterone binding method and by an electroim-munoassay technique. The mean serum concentration of SHBG in healthy, regularly menstruating women (n=42) was 3.7±1.0 (SD, standard deviation) mg/1 and in healthy men (n=100) 2.0±0.9 mg/1.Keywords
This publication has 23 references indexed in Scilit:
- Current status of the molecular structure and function of the plasma sex steroid-binding protein (SBP)Journal of Steroid Biochemistry, 1983
- Transport of Steroid Hormones: Binding of 21 Endogenous Steroids to Both Testosterone-Binding Globulin and Corticosteroid-Binding Globulin in Human PlasmaJournal of Clinical Endocrinology & Metabolism, 1981
- A simple two-step procedure for the simultaneous isolation of corticosteroid binding globulin and sex hormone binding globulin from human serum by chromatography on cortisol-sepharose and phenyl-sepharoseThe Journal of Steroid Biochemistry and Molecular Biology, 1981
- Transport of Protein-Bound Hormones into Tissuesin Vivo*Endocrine Reviews, 1981
- Androgen Transport Proteins: Physical Properties, Hormonal Regulation, and Possible Mechanism of TeBG and ABP ActionArchives of Andrology, 1981
- Relative Binding of Testosterone and Estradiol to Testosterone-Estradiol-Binding Globulin*Journal of Clinical Endocrinology & Metabolism, 1979
- Further characterization and immunological studies of human sex steroid binding plasma proteinJournal of Steroid Biochemistry, 1979
- Purification and characterization of human sex hormone binding globulinThe Journal of Steroid Biochemistry and Molecular Biology, 1979
- SEX‐HORMONE‐BINDING GLOBULINClinical Endocrinology, 1974
- A Simplified Method for the Quantitative Determination of Testosterone-Estradiol-Binding Globulin Activity in Human PlasmaJournal of Clinical Endocrinology & Metabolism, 1972