Localization of the major dehydrogenases in two methylotrophs by radiochemical labeling

Abstract

The localization of prominent proteins in intact cells of 2 methylotrophic bacteria, Hyphomicrobium sp. strain X and bacterium W3A1, was investigated by radiochemical labeling with [14C]isethionyl acetimidate. In bacterium W3A1, trimethylamine dehydrogenase was not labeled by the reagent and is thus an intracellular protein; the periplasmic location of the methylamine and methanol dehydrogenases was evidenced by being readily labeled in intact cells. An intracellular location of the trimethylamine and dimethylamine dehydrogenases in Hyphomicrobium sp. strain X was indicated; methanol dehydrogenase was periplasmic.