Structure of the galactokinase gene ofEscherichia coli, the last (?) gene of thegaloperon

Abstract

We present the nucleotide sequence of the galactokinase gene ( gal K) of Escherichia coli including its 5′ and 3′ flanking regions. This DNA sequence derives from the λga18 transducing phage and is identical to the sequence present in the gal k gene fusion vectors, pKO and pKG, commonly used to study transcriptional regulatory elements. We define the precise 3′ junction between the bacterial and phage sequences in λga18 and demonstrate that this junction prohably results from a homologous recombination event between identical 9 bp sequences common to the gal operon and phage λ. Moreover, we examine the 300 bp region located immediately beyond gal K for transcription termination function and find no gal operon terminator. Lastly, we compare the gal K genes of E . coli and the yeast S . cerevisiae and find several regions of strong homology among which is a potential ATP-binding site homology shared by a variety of ATP-binding proteins including protein kinases encoded by mammalian oncogenes.