Characterization of the cell cycle and synchrony in a CV-1 cell line

Abstract

Synchronous populations of CV‐1 cells have been prepared by the selective detachment of mitotic cells. An approach, using a combination of standard techniques, has allowed evaluation of the degree of synchrony throughout the cell cycle. CV‐1 cells have a cycle of ∼19‐hr duration, throughout which synchronous populations progress with a dispersion of 3 hr, i.e., ≥90% of the cells enter S phase and subsequently divide within a 3‐hr period. A major cause of this dispersion is the differential attachment times of individual cells within the population when a synchronous culture is initiated.