Mediation of Delayed Hypersensitivity by Peripheral Blood Lymphocytes in Vitro and by Their Products in Vivo and in Vitro, Morphology of in Vitro Lymphocyte-Macrophage Interaction

Abstract

Peripheral blood lymphocytes from tuberculin hypersensitive guinea pigs and mediating material that these cells produced in the presence of specific antigen altered the activity (migratory potential) of normal macrophages. The mediating material produced by such peripheral lymphocytes caused enhanced tuberculin skin reactions. Its in vitro and in vivo influence on delayed hypersensitivity was inactivated by storage at 4°C and -20°C. Unique associations of lymphocytes and macrophages regularly appeared in cultures of sensitive peritoneal exudate cells, as well as in cultures of mixtures of sensitive peripheral blood lymphocytes with normal peritoneal exudate cells, when specific antigen was in the medium.