Bacterial adhesion to poly(HEMA)‐based hydrogels
- 1 January 1993
- journal article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 27 (1), 119-126
- https://doi.org/10.1002/jbm.820270115
Abstract
The effects of water content and comonomer chemistry upon the adhesion of Pseudomonas aeruginosa to poly(hydroxyethyl methacrylate)‐based hydrogels were studied. Hydrogels which varied in swollen water content from 33–69 wt% were polymerized onto glass microscope slides pretreated with a vinyl silane. The hydrogel water content was varied by adding methacrylic acid (1–5 wt%) orN‐vinyl pyrrolidone (NVP, 10–25 wt%) or combinations of the two comonomers. The resulting hydrogel surfaces, which were 0.1 mm thick, transparent, and adherent to the glass slide, formed the test surfaces of laminar flow cells (Re = 1.3, wall shear rate = 1.6/s). The bacteria were grown for 8 h in tryptic soy broth (TSB), washed by filtration, and collected on 0.45‐μm filters, resuspended in phosphate buffered saline (PBS) at pH = 7.2, and recirculated through the flow cell and across the test surface at 0.85 mL/min for 2 h. Results show that P. aeruginosa adhered less to hydrogels with higher water contents. In the presence of TSB and possible poly(NVP) contamination, the concentration of adherent bacteria was reduced to low and uniform levels independent of the hydrogel chemistry. © 1993 John Wiley & Sons, Inc.Keywords
This publication has 18 references indexed in Scilit:
- Contact lens surface deposits increase the adhesion of Pseudomonas aeruginosaCurrent Eye Research, 1990
- The role of bacterial hydrophobicity in infection: bacterial adhesion and phagocytic ingestionCanadian Journal of Microbiology, 1988
- Extended-Wear Lenses, Biofilm, and Bacterial AdhesionArchives of Ophthalmology (1950), 1987
- Pseudomonas Attachment to New Hydrogel Contact LensesArchives of Ophthalmology (1950), 1987
- Measurement of contact angles on biological and other highly hydrated surfacesJournal of Colloid and Interface Science, 1986
- Adhesion of coagulase‐negative staphylococci to methacrylate polymers and copolymersJournal of Biomedical Materials Research, 1986
- The Pathogenesis of Contact Lens-Associated Pseudomonas aeruginosa Corneal UlcerationCornea, 1986
- Corneal Ulcers Associated With Contact Lens WearArchives of Ophthalmology (1950), 1984
- Measurement of the polar force contribution to adhesive bondingJournal of Colloid and Interface Science, 1974