Nature of the Nets Produced by Protoplasts of Schizosaccharomyces pombe During the First Stage of Wall Regeneration in Liquid Media

Abstract

The microfibrillar nets formed during the 1st stage of wall regeneration by protoplasts of S. pombe in a liquid medium contain, in contrast to normal walls, (1 .fwdarw. 3)-.beta.-linked glucan of high crystallinity, like the nets produced by protoplasts of Saccharomyces cerevisiae under the same conditions. During wall regeneration by protoplasts of Schizosaccharomyces, .beta.-glucan synthesis precedes the formation of (1 .fwdarw. 3)-.alpha.-glucan.