Bunyamwera virus-induced polypeptide synthesis

Abstract

Bunyamwera virus-induced polypeptide synthesis in [African green monkey kidney] BSC-1 cells was studied using polyacrylamide gel electrophoresis and autoradiography. Four virus-induced polypeptides were identified. Their MW were 200 .times. 103 (L), 128 .times. 103 (G1), 31 .times. 103 (G2) and 23 .times. 103 (N). Pulse-chase experiments, short labeling experiments and experiments using amino acid analogs failed to show evidence of polypeptide processing by proteolytic cleavage. Analysis of the kinetics of synthesis of these polypeptides showed that a clear division into early and late categories could be made, the onset of synthesis of polypeptide N preceding that of polypeptides G1 and G2. The rate of synthesis of polypeptides N and L rapidly reached a peak and then declined. Polypeptides G1 and G2 were made for several hours; their rate of synthesis then declined. All 4 polypeptides then continued to be made in relatively small amounts for many hours.