ISOLATION OF PANCREATIC ISLETS FROM DOGS
- 1 August 1990
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 50 (2), 207-209
- https://doi.org/10.1097/00007890-199008000-00007
Abstract
We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell precessor. Islet preparations have a purity of 86.5% and contain 36.8% .mu.l of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following autotransplantation.This publication has 4 references indexed in Scilit:
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- BOVINE SERUM ALBUMIN DENSITY GRADIENT ISOLATION OF RAT PANCREATIC ISLETS1Transplantation, 1987
- Natural history of intrahepatic canine islet cell autografts.Journal of Clinical Investigation, 1986
- USE OF FICOLL IN SEPARATION OF VIABLE ISLETS OF LANGERHANS FROM RAT PANCREAS1976