Induced mitotic crossing-over in relation to genetic replication in synchronously dividing cells ofUstilago maydis

Abstract

A method is now available for synchronizing the division of populations of cells of the smut fungusUstilago maydis. In two experiments carried out with a diploid strain heterozygous for several biochemical markers, samples of cells were removed at intervals through the synchronized division cycle and treated with a constant dose of ultra-violet light. Cell survival and the frequency of the various recombinants resulting from induced mitotic crossing-over were recorded. In addition the period of DNA synthesis in the unirradiated population has been measured. During this period the cells are very sensitive to ultra-violet light and those that survive contain the highest proportion of induced recombinants. In so far as the markers make it possible to locate the position of cross-overs, cells which are irradiated early in the period of genetic replication show most crossing-over towards the ends of the chromosome arms, whilst cells which are treated late in this period show crossing-over near the centromeres. The data are most easily interpreted by supposing that chromosome replication begins at the ends of the arms and proceeds to the centromere, and that the temporary interruption of this process by ultra-violet light can result in pairing and crossing-over in the vicinity of the points of interruption.