Structural and Functional Properties of Plasma Membranes from the Filamentous Fungus Penicillium chrysogenum
Open Access
- 1 September 1994
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 224 (2), 581-587
- https://doi.org/10.1111/j.1432-1033.1994.t01-1-00581.x
Abstract
Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps, namely homogenization of cells with a Braun MSK homogenizer, followed by Percoll gradient centrifugation and floatation of membranes in a three-step Nycodenz gradient. This method can be applied to strains which differ significantly in morphology and penicillin-production capacity. Plasma membranes were fused with liposomes containing the beef heart mitochondria1 cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes maintained a high proton motive force that functions as a driving force for the uptake of the amino acids arginine and valine via distinct transport systems.Keywords
This publication has 31 references indexed in Scilit:
- Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markersJournal of General Microbiology, 1992
- Evidence for a compartmentation of penicillin biosynthesis in a high- and a low-producing strain of Penicillium chrysogenumJournal of General Microbiology, 1991
- Metabolism and compartmentation of α-aminoadipic acid in penicillin-producing strains of Penicillium chrysogenumBiochimica et Biophysica Acta (BBA) - General Subjects, 1989
- ATP‐driven Ca2+ transport in sealed plasma membrane vesicles prepared by aqueous two‐phase partitioning from leaves of Commelina communisPhysiologia Plantarum, 1989
- Composition ofNeurospora crassa vacuolar membranes and comparison to endoplasmic reticulum, plasma membranes, and mitochondrial membranesExperimental Mycology, 1987
- Amino acid transport in eucaryotic microorganismsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1986
- Induction of penicillin biosynthesis by L-glutamate in Penicillium chrysogenumBiochemical and Biophysical Research Communications, 1982
- Plant Cell FractionationAnnual Review of Plant Physiology, 1979
- Phosphotungstic Acid-Chromic Acid as a Selective Electron-Dense Stain for Plasma Membranes of Plant CellsStain Technology, 1972
- A METHOD FOR THE QUANTITATIVE ESTIMATION OF CYTOPLASMIC STRUCTURESThe Journal of cell biology, 1962