Detection of Wheat Spindle Streak Mosaic Virus by Serologically Specific Electron Microscopy

Abstract

Wheat spindle streak mosaic virus was readily detected by serologically specific electron microscopy (SSEM) in wheat [Triticum aestivum cv. Ionia] plants suspected to be infected with this virus. About 1000 times more particles were observed in crude sap from plants grown in the field and in a growth chamber by SSEM than by conventional transmission electron microscopy. Particles were 16 nm in diameter and ranged 600-3800 nm long, with a modal length of 1775 nm. SSEM provides a routine rapid assay for detecting WSSMV-infected plants.