A role for sialyl Lewis-X/A glycoconjugates in capillary morphogenesis
- 1 September 1993
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 365 (6443), 267-269
- https://doi.org/10.1038/365267a0
Abstract
TO identify cell adhesion molecules required for angiogenesis, we used an in vitro model in which bovine capillary endothelial cells can be induced to form capillary-like tubes. Monoclonal antibodies directed against the carbohydrate epitopes sialyl Lewis-X and sialyl Lewis-A inhibited capillary formation. We postulated that a member of the selectin family of adhesion molecules may be involved in capillary formation because these proteins bind to sialyl Lewis-X/A-containing ligands. We isolated a 2.8-kilobase comple-mentary DNA from a bovine capillary endothelial cell cDNA lib-rary which encodes a polypeptide with 71% identity to human E-selectin. We report here that antibody directed against the bovine E-selectin inhibited capillary formation, suggesting that in addition to its role in leukocyte adhesion to endothelium, a form of E-selectin is involved in capillary morphogenesis.Keywords
This publication has 27 references indexed in Scilit:
- Isolation and Characterization of a Bovine cDNA Encoding a Functional Homolog of Human P-SelectinBiochemical and Biophysical Research Communications, 1993
- Selectins.JCI Insight, 1993
- The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells.The Journal of cell biology, 1991
- Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis ABiochemical and Biophysical Research Communications, 1991
- Recognition by Elam-1 of the Sialyl-Le x Determinant on Myeloid and Tumor CellsScience, 1990
- Autoradiography using storage phosphor technologyElectrophoresis, 1990
- Endothelial Leukocyte Adhesion Molecule 1: an Inducible Receptor for Neutrophils Related to Complement Regulatory Proteins and LectinsScience, 1989
- Angiogenic FactorsScience, 1987
- Induction and detection of a human endothelial activation antigen in vivo.The Journal of Experimental Medicine, 1986
- Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigensNature, 1985