Post-testicular change in the reptile sperm surface with particular reference to the snake,Natrix fasciata
- 1 January 1987
- journal article
- research article
- Published by Wiley in Journal of Experimental Zoology
- Vol. 241 (1), 123-132
- https://doi.org/10.1002/jez.1402410115
Abstract
Sperm surface changes occurring in the reptile Wolffian duct have been explored with particular references to the snake, Natrix fasciata. In the snake Wolffian duct there are several proteins not present in serum, the pattern of which changes in concert with the seasonal testicular cycle. Whereas testicular spermatozoa did not bind antibody to duct secretions, all Wolffian duct spermatozoa did so over both head and tail, according to immunofluorescence patterns. Thus, on entering the Wolffian duct, the entire surface of N. fasciata spermatozoa acquires one of more of the duct's secretory components. As indicated by immunofluorescence, immunoelectrophoresis, and immunodiffusion, epitopes on at least some molecules that bind to spermatozoa or that remain free in the duct fluid are shared with those in other Natrix species, but not in more distant reptiles (turtle, anole lizard), nor chicken, rat, or rabbit. In regard to glycoproteins, one prominent con A‐reactive band was present in polyacrylamide gel electrophoresis (PAGE) of snake fluid and more were evident in fluid collected from the turtle duct. However, such lectin‐reactive elements did not bind to spermatozoa as judged by an absence of any change in snake, turtle and lizard sperm lectin‐binding patterns in passing from the testis into and through the Wolffian duct. In all, evidence from these and other species studied begins to suggest that the nature of the post‐testicular sperm surface modification displayed in most vertebrates that fertilize internally may differ in sub‐therian and therian groups, respectively. There appears to be a relative emphasis on glycosyl‐rich surface elements in the latter. The possible significance of these changes for sperm function in the different groups is discussed briefly in terms of sperm survival/storage, as well as capacitation and sperm binding to the zona.This publication has 15 references indexed in Scilit:
- Surface of the rooster spermatozoon changes in passing through the Wolffian ductJournal of Experimental Zoology, 1985
- Lactosaminoglycans synthesized by mouse male germ cells are fucosylated by an epididymal fucosyltransferaseDevelopmental Biology, 1984
- Sperm maturation, fluid transport, and secretion and absorption of protein in the epididymis of the echidna, Tachyglossus aculeatusReproduction, 1983
- Significance of the Need for Sperm Capacitation Before Fertilization in Eutherian MammalsBiology of Reproduction, 1983
- Binding to spermatozoa of a major soluble protein secreted by the epididymis of the lizard Lacerta viviparaGamete Research, 1983
- UDP-Galactose: N-Acetylglucosamine Galactosyltransferase in Fluids from Rat Rete Testis and Epididymis1Biology of Reproduction, 1980
- An evolutionary view of the male reproductive tract and sperm maturation in a monotreme mammal ‐ the echidna, Tachyglossus aculeatusJournal of Anatomy, 1979
- Lipid changes in boar spermatozoa during epididymal maturation with some observations on the flow and composition of boar rete testis fluidReproduction, 1979
- Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectinsAnalytical Biochemistry, 1979
- An Examination for Sperm Capacitation in the Fowl1Biology of Reproduction, 1970