HUMAN CHROMOSOMAL ASSIGNMENTS FOR 14 ARGININOSUCCINATE SYNTHETASE PSEUDOGENES - CLONED DNAS AS REAGENTS FOR CYTOGENETIC ANALYSIS
- 1 January 1984
- journal article
- research article
- Vol. 36 (5), 954-964
Abstract
There are multiple, processed, dispersed pseudogenes for human argininosuccinate synthetase. Chinese hamster .times. human somatic cell hybrids were used to map DNA fragment groups corresponding to the single expressed gene and 14 pseudogene loci. Each chromosomal assignment was confirmed using hybrids containing very few human chromosomes and/or by demonstrating monosomic or trisomic dosage in human cell lines with chromosomal abnormalities. Pseudogenes were mapped to chromosomes 2cen-p25, 3q12-qter, 4q21-qter, 5 (2 loci), 6, 7, 9p13-q11, 9q11-q22, 11q, 12, Xp22-pter, Xq22-q26 and Ycen-q11. DNA fragments from the expressed gene were mapped to 9q34-qter in agreement with the previous assignment for enzyme activity. A high-frequency restriction fragment length polymorphism mapped to 9q11-q22. The analyses emphasized the feasibility of using chromosomally abnormal human cell lines for confirmation and regionalization of gene-mapping assignments made using somatic-cell hybrids. Cloned DNA probes, once mapped and characterized, can be very valuable for determining the chromosomal composition of interspecies hybrids and the dosage of loci in human cells. The argininosuccinate synthetase c[complementary]DNA is a convenient reagent for dosage analysis of 15 human loci on 11 different chromosomes. Improved reagents could be designed that would simplify South blot patterns by eliminating overlapping DNA fragments and providing a single DNA fragment for each locus.This publication has 12 references indexed in Scilit:
- A three-allele restriction-fragment-length polymorphism at the hypoxanthine phosphoribosyltransferase locus in man.Proceedings of the National Academy of Sciences, 1983
- Dispersion of argininosuccinate synthetase-like human genes to multiple autosomes and the X chromosomeCell, 1982
- Sequences on the human Y chromosome homologous to the autosomal gene for argininosuccinate synthetaseNature, 1982
- Cloning of cDNA for argininosuccinate synthetase mRNA and study of enzyme overproduction in a human cell line.Journal of Biological Chemistry, 1981
- Deletions of Chromosome 15 as a Cause of the Prader–Willi SyndromeNew England Journal of Medicine, 1981
- Transformation of mammalian cells with genes from procaryotes and eucaryotesCell, 1979
- REGIONAL LOCALIZATION OF HUMAN-GENE LOCI ON CHROMOSOME-9 - STUDIES OF SOMATIC-CELL HYBRIDS CONTAINING HUMAN TRANSLOCATIONS1979
- Regional assignments of the loci AK3, ACONs, and ASS on human chromosome 9Cytogenetic and Genome Research, 1979
- Simultaneous identification of chromatid replication and of human chromosomes in metaphases of man-mouse somatic cell hybridsCytogenetic and Genome Research, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975