Extracellular phospholipase A2 activity in cell free peritoneal lavage fluid from mice with zymosan peritonitis

Abstract
The kinetics and appearance of extracellular phospholipase A2 (PLA2) activity and its relationship to the appearance of arachidonic acid (AA) metabolites in the peritoneal cavity of mice injected with zymosan is described. AA metabolites levels including leukotriene C4 (LTC4) were maximum 15–30 min after zymosan. Peak PLA2 activity was also found 15–30 min after zymosan and was significantly increased above levels found in saline control exudates (3.83±0.89 and 0.35±0.11, respectively,p≤0.05). Results show that an extracellular PLA2 is present in zymosan peritonitis.