Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.
- 1 July 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (13), 4010-4014
- https://doi.org/10.1073/pnas.79.13.4010
Abstract
The kinetics of the interaction between endodeoxyribonuclease EcoRI (EC 3.1.23.13) and 9 linear DNA fragments that range in size between 34 and 6200 base pairs and contain the EcoRI site of plasmid pBR322 in a central location were examined. The kinetic parameters governing both formation and decay of specific endonuclease.cntdot.DNA complexes increase 8-fold with increasing chain length over this size range. Equilibrium competition experiments demonstrated that the intrinsic affinity of endonuclease for its recognition sequence is independent of DNA chain length over this range. DNA sequences outside the recognition site enhance the rate at which EcoRI endonuclease locates or leaves its recognition site without affecting the intrinsic thermodynamic parameters of site-specific interaction. These results are consistent with a facilitated diffusion mechanism for specific DNA site location by this enzyme.This publication has 27 references indexed in Scilit:
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