Mutants of Chinese hamster ovary cells with altered glucose-6-phosphate dehydrogenase activity
- 1 May 1977
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 3 (3), 323-333
- https://doi.org/10.1007/bf01538750
Abstract
Two mutant clones of a Chinese hamster ovary cell line deficient in glucose-6-phosphate dehydrogenase (G6PD) activity have been characterized. In each case, there is evidence that a structural gene mutation has taken place. The first mutant produces 11% specific enzyme activity compared to wild-type parental cells, but this residual activity is much more heat sensitive than that of the wild type. The second mutant contains no residual activity, but a revertant was isolated that exhibits a partial restoration of G6PD activity with, again, an increased heat sensitivity. The selection of G6PD* cells from G6PD}-populations can be effected by exploiting the increased sensitivity of the latter to diamide, a compound that depletes the cell of reduced glutathione.Keywords
This publication has 16 references indexed in Scilit:
- Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cellsSomatic Cell and Molecular Genetics, 1976
- Carbohydrate preferences of mammalian cellsJournal of Cellular Physiology, 1976
- PARASEXUAL APPROACHES TO THE GENETICS OF MANAnnual Review of Genetics, 1975
- Human erythrocyte glucose 6-phosphate dehydrogenase. Interaction with oxidized and reduced coenzymeBiochemical and Biophysical Research Communications, 1974
- Human erythrocyte glucose 6-phosphate dehydrogenase. Content of bound coenzymeBiochemical and Biophysical Research Communications, 1974
- X‐CHROMOSOME INACTIVATION AND DEVELOPMENTAL PATTERNS IN MAMMALSBiological Reviews, 1972
- Amino acid substitution (histidine to tyrosine) in a glucose-6-phosphate dehydrogenase variant (G6PD Hektoen) associated with over-productionJournal of Molecular Biology, 1970
- EVOLUTION OF SEX CHROMOSOMES IN MAMMALSAnnual Review of Genetics, 1969
- Lest I Forget Thee, Glutathione …Nature, 1969
- Erythrocyte Glucose-6-Phosphate Dehydrogenase in Caucasians: New Inherited VariantScience, 1964