The Separation of Willebrand Factor from Factor VIII-Related Antigen

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Abstract
The 3 activities associated with factor VIII.sbd.coagulant (VIII:C), antigenic (VIIIR:Ag) and platelet agglutinating or Willebrand facot (VIIIR:WF).sbd.were separated by sequential antibody affinity chromatography, utilizing a rabbit antibody to factor VIII and a spontaneous human antibody to VIII:C. Normal plasma differentially lost its factor VIII-related antigen following passage over the rabbit antibody column. Subsequent passage of the VIIIR:Ag-depleted plasma over the human antibody column resulted in the loss of VIII:C activity, with retention of the Willebrand factor activity, antigen being partially recovered from the heterologous antibody column. It is possible to separate 2 of the factor VIII activities, VIIIR:Ag and VIIIR:WF, which are usually regarded as properties of 1 molecule.