A novel cyclic GMP-lowering agent, LY83583, blocks carbachol-indiiced cyclic GMP elevation in rabbit atrial strips without blocking the negative inotropic effects of carbachol
- 1 August 1985
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Physiology and Pharmacology
- Vol. 63 (8), 908-911
- https://doi.org/10.1139/y85-150
Abstract
A novel cyclic GMP-lowering agent, LY83583(6-anilino-5,8-quinolinedkme), was used to investigate the possibility that increases in myocardial cyclic GMP levels are responsible for the negative inotropic effects of cholinergic agonists. Concentrations of carbachol from 0.3 to 3 μM elevated cyclic GMP levels in electrically paced rabbit atrial strips by 75 to 200% and decreased contractile force in the strips by 30 to 60%. Pretreatment of the muscles for 10 min with 10 μM LY83583 significantly lowered resting cyclic GMP levels and completely blocked the elevation of cyclic GMP by these concentrations of carbachol. However, the negative inotropic effects of carbachol were not blocked by the LY83583. These results indicate that the negative inotropic effects of carbachol in rabbit atrium are not mediated by increases in tissue levels of cyclic GMP.This publication has 11 references indexed in Scilit:
- PHARMACOLOGIC ANALYSIS OF 2 NOVEL INHIBITORS OF LEUKOTRIENE (SLOW REACTING SUBSTANCE) RELEASE1984
- Inotropic responses of the frog ventricle to dibutyryl cyclic AMP and 8-bromo cyclic GMP and related changes in endogenous cyclic nucleotide levelsBiochemical Pharmacology, 1981
- Inotropic responses of the frog ventricle to adenosine triphosphate and related changes in endogenous cyclic nucleotides.The Journal of Physiology, 1980
- EFFECTS OF ACETYLCHOLINE AND NITROPRUSSIDE ON CGMP-DEPENDENT PROTEIN-KINASE IN THE PERFUSED RAT-HEART1980
- Ethanol inhibition of antigen-induced histamine release from guinea pig lung: Correlation with intracellular levels of cyclic nucleotidesLife Sciences, 1979
- Cyclic nucleotides and cardiac function.Circulation Research, 1979
- On the question of cyclic GMP as the mediator of the effects of acetylcholine on the heartBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Are increases in cyclic GMP levels responsible for the negative inotropic effects of acetylcholine in the heart?Biochemical and Biophysical Research Communications, 1977
- DISSOCIATION OF CYCLIC-GMP FROM NEGATIVE INOTROPIC ACTION OF CARBACHOL IN GUINEA-PIG ATRIA1977
- Negative inotropic effect of cyclic GMP in cardiac fiber fragmentsPflügers Archiv - European Journal of Physiology, 1976