Diversity and Complexity of Uterine mRNA from Rats of Differing Hormonal States
- 1 October 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 100 (1), 85-94
- https://doi.org/10.1111/j.1432-1033.1979.tb02036.x
Abstract
The diversity and complexity of the uterine mRNA population was compared at 2 different stages of uterine growth and development in the rat. Analysis by c[complementary]DNA hybridization to homologous mRNA indicated that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of estradiol-17.beta.-induced growth while the fully developed uterus expresses 36,000 sequences. As a check on the results, the complexity was re-analyzed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicated that the hormone-stimulated immature rat uterus contains 12,000 poly(A)-containing mRNA sequences while the fully differentiated adult tissue contains 53,000 diverse sequences.This publication has 41 references indexed in Scilit:
- Oestrogen-Induced Changes in the Population of Uterine Polyribosomal Polyadenylated RNABiochemical Society Transactions, 1978
- Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17β on its synthesisBiochemical Journal, 1978
- The expression of the vitellogenin geneCell, 1976
- Steroid Receptors: Elements for Modulation of Eukaryotic TranscriptionAnnual Review of Biochemistry, 1976
- Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNAAnalytical Biochemistry, 1976
- Current models of steroid hormone action: a critique.Annual Review of Physiology, 1976
- The effects of oestradiol-17β on the ribonucleic acid polymerases of immature rabbit uterusBiochemical Journal, 1975
- Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acidJournal of Molecular Biology, 1974
- The synthesis of ribonucleic acid in immature rat uterus responding to oestradiol-17βBiochemical Journal, 1971
- Regulation of polyribosome formation and protein synthesis in the uterus. Effect of ovariectomy and administration of oestradiol-17β on the amino acid-incorporation activity in vitro and the cytoplasmic concentration in vivo of polyribosomal preparationBiochemical Journal, 1967