The effect of anticomplementary substances on properdin in normal and C2-deficient sera.
- 1 March 1975
- journal article
- Vol. 19 (3), 435-44
Abstract
The effect of anticomplementary substances, including zymosan, cobra venom, endotoxin, inulin and immune complexes, on serum properdin concentration and immunoelectrophoretic mobility was studied. In normal serum, zymosan, inulin and immune complexes 'fixed' properdin, while in C2-deficient serum, only zymyosan 'fixed' properdin. Slowly migrating properdin (P) was detected in normal serum following activation by endotoxin and cobra venom but in C2-deficient serum only with cobra venom. Endotoxin did not activate the alternative pathway proteins studied in C2-deficient serum. Fast migrating properdin (P) represented activated properdin and occured as a result of activation of properdin in the Noble agar medium used for electrophoresis provided sufficient cofactors, including Mg2+, were present.This publication has 19 references indexed in Scilit:
- The Utilization of Properdin in the Alternate Pathway of Complement Activation: Isolation of Properdin ConvertaseThe Journal of Immunology, 1974
- C2 Deficiency DEVELOPMENT OF LUPUS ERYTHEMATOSUSJCI Insight, 1973
- Properdin and C3 Proactivator: Alternate Pathway Components in Human GlomerulonephritisJCI Insight, 1973
- Determination of Serum Properdin Levels by ImmunodiffusionInternational Archives of Allergy and Immunology, 1973
- The Immunoelectrophoretic Pattern of Properdin in Fresh and Aged Human SerumExperimental Biology and Medicine, 1972
- Opsonic Activity in Human Serum Deficient in C2The Journal of Immunology, 1972
- C3 PROACTIVATOR CONVERTASE AND ITS MODE OF ACTIONThe Journal of Experimental Medicine, 1972
- INTERACTIONS OF THE COMPLEMENT SYSTEM WITH ENDOTOXIC LIPOPOLYSACCHARIDE: CONSUMPTION OF EACH OF THE SIX TERMINAL COMPLEMENT COMPONENTSThe Journal of Experimental Medicine, 1968
- Properties of Highly Purified Human ProperdinThe Journal of Immunology, 1968
- THE PROPERDIN SYSTEM AND IMMUNITYThe Journal of Experimental Medicine, 1956