Trichloressigsäure-Aceton-Extraktion von Albuminen aus Seren und Antigen-Antikörper-Präzipitaten

Abstract

Highly purified albumin fractions can be obtained by precipitating human, rabbit, or rat sera with trichloracetic acid, and extracting the precipitates with acetone. Human albumin thus prepared is identical with the original albumin in its electrophoretic properties, its dye-binding ability, and in its im-munological behavior. After precipitating human albumin with antibodies the albumin antigen can be extracted in a good yield by use of trichloracetic acid and acetone without losing the electrophoretic or antigenic characteristics of albumin.