Heterogeneity among flavivirus proteins separated in slab gels

Abstract

³⁵S-methionine-labeled proteins specified in Vero cells by flaviviruses were analysed by SDS-phosphate electrophoresis in polyacrylamide slab gels. The clarity of the profile produced by Kunjin virus permitted designation of the non-structural proteins, and confirmed the identity of NV 2 1/2 the profile included a protein previously designated V 2 (core protein) but now named NV 1 1/2 because it migrates perceptibly faster than V 2. Despite a varying background of labeled host proteins, identifiable profiles were obtained for 11 of 12 flaviviruses. The large non-structural proteins NV 5 and NV 4 migrated at apparently the same rates for all viruses. Profiles of the remaining proteins displayed varying amounts of heterogeneity, notably in the migration of the envelope protein V 3 which showed no evidence of subgroup specificity.