‘Binding’ of glycine andγ-aminobutyric acid to synaptosomal fractions of 6 regions of the feline brain; effects of strychnine

Abstract

GABA (6×10⁻⁶ M) binding to synaptosome-enriched fractions of cat CNS exhibited a clear rostro-caudal gradient, whereas glycine (6×10⁻⁶ M) binding was greatest to particles of cerebellar cortex, and this was followed by medulla ≅ caudate nucleus ≧ cerebral cortex ≧ pons > corona radiata. Strychnine-SO₄ (10⁻³ or 10⁻⁴ M) inhibited the binding of GABA and glycine in all brain regions studied; at 10⁻⁵ M this drug inhibited the binding of both GABA and glycine only to particles of the cerebral cortex.