USE OF MONOCLONAL-ANTIBODIES TO SIALYLATED LEWIS-X AND SIALYLATED LEWIS-A FOR SEROLOGICAL TESTS OF CANCER
- 1 January 1985
- journal article
- research article
- Vol. 45 (1), 435-437
Abstract
A new monoclonal antibody, CSLEX1, directed against sialylated Lewisx was tested in parallel with a monoclonal antibody, CSLEA1, directed against sialylated Lewisa antigen. In tests with a solid-phase radioimmune sandwich assay, the sialylated Lewisx monoclonal antibody detected sera from certain cancer patients that were negative with the sialylated Lewisa monoclonal antibody. Some sera from cancer patients showed the reverse reaction. The combined use of these 2 monoclonal antibodies detects a wider range of sera from cancer patients that the use of a single antibody alone. It should be possible in the future to use multiple monoclonal antibodies to increase detection.This publication has 9 references indexed in Scilit:
- CHARACTERIZATION OF SIALOSYLATED LEWIS AS A NEW TUMOR-ASSOCIATED ANTIGEN1984
- A Glycolipid Antigen Associated with Burkitt Lymphoma Defined by a Monoclonal AntibodyScience, 1983
- Mouse monoclonal antibodies against human cancer cell lines with specificities for blood group and related antigens. Characterization by antibody binding to glycosphingolipids in a chromatogram binding assay.Journal of Biological Chemistry, 1983
- A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.Journal of Biological Chemistry, 1982
- Monoclonal antibody detection of a circulating tumor-associated antigen. II. A longitudinal evaluation of patients with colorectal cancerJournal of Clinical Immunology, 1982
- Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril (Iodogen)Analytical Biochemistry, 1981
- Cell surface sialic acid expression of lectin-resistant variant clones of B16 melanoma with altered metastasizing potentialBiochemical and Biophysical Research Communications, 1980
- SERUM SIALYLTRANSFERASE LEVELS AS A PARAMETER IN THE DIAGNOSIS AND FOLLOW-UP OF GASTROINTESTINAL TUMORS1980
- Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).Proceedings of the National Academy of Sciences, 1978