IMMUNOPOTENTIATING EFFECTS OF AMPHOTERICIN-B .2. ENHANCED INVITRO PROLIFERATIVE RESPONSES OF MURINE LYMPHOCYTES

Abstract

Enhanced in vitro proliferative responses to DNBSO3 [sodium 2,4-dinitrobenzene-1-sulfonate] were seen in lymph node cells and spleen cells after in vivo sensitization of mice with DNFB [dinitrofluorobenzene] plus AmB [amphotericin B] compared with mice primed with DNFB alone. The T [thymus-derived] cell proliferation in the nylon column non-adherent fraction for both groups was highly similar and the enhanced lymph node cell proliferation with AmB was demonstrated to be in the nylon adherent population consisting of T and B [bone marrow-derived] cells. A mechanism that depends on selective interaction of the polyene with a subset of T cells and resultant impairment of the normally induced suppressor regulation that limits magnitude and duration of immune responses is indicated.