The Use of Soluble Starch Medium in the Preparation of Smooth “O” Salmonella Antigens

Abstract
Rough strains of Salmonella cultures were grown on veal infusion agar containing 2% soluble starch. After one or more subcultures, smooth cultures were produced from these rough strains. Smooth "O" antigens prepd. from these cultures did not show nonspecific granulation. However, these smooth cultures sometimes reverted to the rough form when stored in the cold. The smooth cultures gave a positive reaction to Millon''s reagent and the rough strains a negative reaction.

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