Metabolic Products of AdultClonorchis sinensis: Their Composition and Antigenic Potential
- 1 September 1969
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Helminthology
- Vol. 43 (3-4), 395-402
- https://doi.org/10.1017/s0022149x0000496x
Abstract
AdultClonorchis sinensiswere taken from the bile ducts of infected rabbits and incubated in Ringer's fluid. The soluble metabolic products secreted by the living flukes during incubation were found to include carbohydrate and protein. Enzyme activities of protease, carbohydrases and esterase, but not of lipase, were detected in the samples.Samples with a nitrogen content of 3 μg/ml or more reacted as antigen in immunodiffusion tests in gel against sera of rabbits infected withC. sinensisor immunised against whole fluke extract.The demonstration of enzymes in the metabolic products, the reactions of the samples as antigen and the correlation of antigenic activity with the nitrogen content of the samples suggests that the enzymes detected are effective antigens in clonorchiasis, although complete identity was not demonstrated in this study.The enzymes investigated were those associated with digestion; intracellular enzymes which have less opportunity to elicit antibody production were not investigated.Keywords
This publication has 18 references indexed in Scilit:
- THE NON-ANTIGENICITY OF INTACT OVA OF CLONORCHIS SINENSISJournal of Medical Microbiology, 1969
- THE SITES OF ANTIGEN FORMATION IN DIFFERENT DEVELOPMENTAL STAGES OF CLONORCHIS SINENSISJapanese Journal of Medical Science and Biology, 1969
- Route of entry of Clonorchis sinensis to the mammalian liverExperimental Parasitology, 1968
- THE NATURE AND ORIGIN OF THE FUNCTIONAL ANTIGENS IN HELMINTH INFECTIONSAnnals of the New York Academy of Sciences, 1963
- II. Physiology of immunity to helminth infectionsExperimental Parasitology, 1963
- The physiology of digestion in the larvae of the horse bot-fly,Gasterophilus intestinalis(de Geer)Parasitology, 1958
- STUDIES ON THE MECHANISM OF IMMUNITY IN THE RAT TO THE NEMATODE, NIPPOSTRONGYLUS MURIS12American Journal of Epidemiology, 1953
- Digestion in Parasitic Nematodes. III. The Digestion of ProteinsJournal of Helminthology, 1941
- THE MECHANISM OF ACQUIRED IMMUNITY IN INFECTIONS WITH PARASITIC WORMSPhysiological Reviews, 1940
- STUDIES ON PANCREATIC LIPASE. IPublished by Elsevier ,1936