Megakaryocyte structure and function

Abstract

Recent advances in the understanding of megakaryocyte (MK) function largely have been made through the careful observation of the morphological and structural events underlying MK development. Ultrastructural localization of enzymatic activities has facilitated the specific recognition of their committed diploid precursors. Observation of the sequential features of endomitosis demonstrates that although similar to normal mitosis, cell division aborts at the anaphase stage. The ability of thrombopoietin to induce the full maturation MKs in vitro not only facilitates platelet release but has increased our knowledge of various subcellular aspects of the phenomenon and eventually will improve the in vivo detection of the site of platelet formation and shedding. Finally, the structural and functional consequences of MK molecular dysfunction leading to thrombocytopenia or myelofibrosis can now be investigated because of the development of transgenic animal models. This review aims to incorporate these new findings within the classical knowledge of MK structure related to its function.