Properties of Lubrol-extracted uridine diphosphate glucuronyltransferase
- 1 December 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 125 (4), 991-997
- https://doi.org/10.1042/bj1250991
Abstract
1. A partially purified UDP-glucuronyltransferase was obtained by extracting rat liver microsomal preparations with Lubrol, a non-ionic detergent. 2. The soluble enzyme catalysed conjugation of both o-aminophenol and p-nitrophenol and was extremely stable when compared with untreated microsomal preparations. 3. The characteristics of the conjugation of the two phenols were found to differ with respect to pH optimum, bivalent cation requirement and Michaelis constants, suggesting that more than one enzyme is involved in the conjugation reaction.Keywords
This publication has 24 references indexed in Scilit:
- Partial purification and ouabain sensitivity of lubrol-extracted sodium-potassium transport adenosine triphosphatases from brain and cardiac tissuesBiochemical Pharmacology, 1970
- Partial purification of hepatic UDP-glucuronyltransferase: Studies of some of its propertiesBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- Studies on the activation in vitro of glucuronyltranferaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1969
- Bilirubin conjugation by hepatic microsomes from adult male guinea pigsArchives of Biochemistry and Biophysics, 1968
- Studies of Glucuronidation. IV. Evidences of Different Processes for o-Aminophenol and p-NitrophenolExperimental Biology and Medicine, 1968
- Studies on bilirubin UDP-glucuronyltransferaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1967
- Spontaneous and detergent activation of a glucuronyltransferase in vitroArchives of Biochemistry and Biophysics, 1967
- Variations in glucuronide formation by perinatal liverBiochemical Pharmacology, 1966
- Ethereal and N-linked glucuronide formation by normal and Gunn rats in vitro and in vivoBiochemical and Biophysical Research Communications, 1961
- Enzymatic Synthesis of N-Glucuronic Acid ConjugatesNature, 1957