Foliar flavonoid distribution during Spinacia chloroplast isolation
- 1 April 1981
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 151 (4), 309-313
- https://doi.org/10.1007/bf00393283
Abstract
The flavonoid glycoside accumulation level was compared in class A and in class C spinach chloroplast suspensions. Class A chloroplasts (up to 97% intact) contained about 0.4% of the total flavonoid glycosides present in leaves. Further purification of 97%-intact chloroplast suspensions, through a Percoll gradient, reduced the glycoside level to less than 0.15%. On the other hand, class C chloroplasts (100% broken plastids) contained between 10 to 30 times more flavonoids than intact Percoll purified chloroplasts. These results indicate that chloroplasts could bind vacuolar glycosides during their period of isolation. This hypothesis was confirmed by controlled contamination experiments using a cell-free supernatant as a source of vacuolar glycosides. Furthermore, the level of flavonoids in chloroplasts could be decreased to a level close to that obtained in intact Percoll purified chloroplasts by washing with soluble polyvinylpyrolidone. The results presented in this paper demonstrate the importance of maintaining the physiological integrity of plastids during the course of organelle isolation when investigating flavonic compartmentation in leaves.Keywords
This publication has 13 references indexed in Scilit:
- Repartition des flavonols dans l'epaisseur des feuilles de quelques vegetaux vasculairesPhytochemistry, 1980
- Effet de différents polyphénols sur les mitochondries et les chloroplastes isolésPhytochemistry, 1980
- Untersuchungen zur lokalisation von flavonoiden in piastiden IV. Flavonoidgehalt intakter chloroplasten aus Avena sativa L.Zeitschrift für Pflanzenphysiologie, 1977
- Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica GradientsPublished by Springer Nature ,1977
- Modification of proteins during the oxidation of leaf phenols: Reaction of potato virus X with chlorogenoquinonePhytochemistry, 1977
- The distribution of flavonoids in chloroplasts of twenty five species of vascular plants☆Phytochemistry, 1976
- Identification of Flavonoids and Cinnamic Acid Derivatives from Spinach Chloroplast PreparationsZeitschrift für Naturforschung B, 1972
- Nomenclature for Isolated ChloroplastsNature New Biology, 1972
- Direct and Indirect Transfer of ATP and ADP across the Chloroplast EnvelopeZeitschrift für Naturforschung B, 1970
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949