Phase-Determining Factors in Blastomyces Dermatitidis

Abstract

The yeastlike phase of B. dermatitidis was grown in a chemically-defined semifluid medium, with a mixture of amino acids as the N-source and dextrose as the C-source. Temp. was the most important environmental factor controlling the type of growth. No amino acid, carbohydrate or "growth substance" tested was found to be an essential accessory factor for growth of the yeastlike phase at 37[degree]C. However, the extent of the growth of the yeastlike phase did vary greatly according to the constitution of the medium, while conversion of conidia to yeastlike cells occurred only in specific media.