PURIFICATION AND PROPERTIES OF THE PROTHORACICOTROPIC HORMONE OF THE SILKWORM, BOMBYX MORI
Open Access
- 31 December 1978
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 21 (1), 29-38
- https://doi.org/10.1111/j.1440-169x.1979.00029.x
Abstract
A simple and highly reproducible procedure for partial purification of prothoracicotropic hormone (PTTH) was established starting with 96,000 male adult Bombyx heads. Approximately 28,500-fold purification of PTTH was accomplished with a yield of about 50% and 6 ng of the most purified preparation (“highly purified PTTH”) caused adult development in a brainless Samia pupa. The peptidal nature of PTTH was reconfirmed through the effects of various enzymatic and chemical treatments on the biological activity of “highly purified PTTH”. Gel-filtration indicated the molecular weight of PTTH to be 4,400.This publication has 13 references indexed in Scilit:
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