The comparative immunoreactivities of brain amyloids in Alzheimer's disease and scrapie
- 1 January 1987
- journal article
- research article
- Published by Springer Nature in Acta Neuropathologica
- Vol. 74 (4), 313-323
- https://doi.org/10.1007/bf00687207
Abstract
An antibody was raised to a synthetic peptide corresponding to a published sequence for the first 24 residues of a cerebrovascular amyloid peptide (CVAP). Immunohistochemical staining of tissue sections revealed that the antibody bound extensively to cerebrovascular amyloid in Alzheimer disease (AD/SDAT) and Down's syndrome cases. The antibody bound less extensively to neuritic plaques (primitive and mature) and indetectably to neurofibrillary tangles. The antibody did not label scrapie plaques, scrapie-associated fibrils, or Gerstmann-Sträussler syndrome plaques. Immunoblotting experiments showed that the cerebrovascular amyloid peptide epitopes contaminating the neurofibrillary tangle preparations could be extracted with urea, leaving the neurofibrillary tangles intact. These data confirm that the cerebrovascular amyloid peptide is a component of cerebrovascular amyloid, and suggest that its epitopes are also components of neuritic plaque amyloid. The reduced level of immunostaining on amyloid cores in tissue sections suggests that either the cerebrovascular amyloid peptide epitopes are a minor component of amyloid cores, or that their mode of packing or state of processing in amyloid cores renders them relatively inaccessible to the antibody. We also conclude that the cerebrovascular amyloid peptide is not a component of neurofibrillary tangles. The synthetic cerebrovascular amyloid peptide possesses amyloid-like properties: at neutral pH it forms insoluble aggregates consisting of 5–7-nm fibrils, which form red-green birefringent adducts with Congo red and fluoresce with thioflavine S.Keywords
This publication has 29 references indexed in Scilit:
- In vitro formation of amyloid fibrils from two synthetic peptides of different lengths homologous to alzheimer's disease β-proteinBiochemical and Biophysical Research Communications, 1986
- Isolation and Partial Characterization of Neurofibrillary Tangles and Amyloid Plaque Core in Alzheimerʼs DiseaseJournal of Neuropathology and Experimental Neurology, 1986
- Monoclonal antibodies raised against a subsequence of senile plaque core protein react with plaque cores, plaque periphery and cerebrovascular amyloid in Alzheimer's diseaseNeuroscience Letters, 1986
- Automated Chemical Synthesis of a Protein Growth Factor for Hemopoietic Cells, Interleukin-3Science, 1986
- Alzheimer's disease and Down's syndrome: Sharing of a unique cerebrovascular amyloid fibril proteinBiochemical and Biophysical Research Communications, 1984
- Alzheimer's disease: Initial report of the purification and characterization of a novel cerebrovascular amyloid proteinBiochemical and Biophysical Research Communications, 1984
- The isolation and amino acid composition of senile plaque core proteinBrain Research, 1983
- Amyloid Deposits and AmyloidosisNew England Journal of Medicine, 1980
- Amyloid Deposits and AmyloidosisNew England Journal of Medicine, 1980
- [4] High-voltage paper electrophoresisPublished by Elsevier ,1967