HLA-DR genotyping by restriction fragment length polymorphism analyses
- 1 September 1985
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 22 (3), 231-239
- https://doi.org/10.1007/bf00404482
Abstract
We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.Keywords
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