Different Mobilization of Calcium in Endothelin-1 -Induced Contractions in Human Arteries and Veins: Effects of Calcium Antagonists

Abstract

We studied the role of extra- and intracellular Ca2+ in endothelin-1-induced contractions of the isolated human internal mammary artery and vein. Veins were more sensitive to the peptide than arteries (concentration shift: 3.2-fold; n = 4-10, p < 0.05). The Ca2+ antagonists darodipine, verapamil, and diltiazem (10-7-10-6 M) did not prevent the response to endothelin-1 in both vessels. In contrast, darodipine (10-8-10-6 M), added after the contraction had developed partially reversed the response in the artery (26 .+-. 7%) and particularly in the vein (67 .+-. 5%; n= 4, p < 0.005 compared to the artery). Removal of extracellular Ca2+ reduced the contractions to endothelin-1 (10-8 M) in the artery (control: 89 .+-. 4% of 100 mM KCl; Ca2+-free: 68 .+-. 4% n = 4-6, p < 0.01), but not in the vein except at low concentrations (10-9 M) of the peptide. After removal of intracellular Ca2+ with caffeine in the artery, endothelin-1 still evoked a contraction (17 .+-. 3%, n = 3; p < 0.005 vs. control), while in the vein the response was abolished. Thus, mobilization of Ca2+ during endothelin-1-induced contractions differs in the human internal mammary artery and vein. In the artery, the contraction depends on extracellular Ca2+, intracellular caffeine-sensitive Ca2+ stores, and a caffeine-insensitive component, while in veins, mobilization of intracellular Ca2+ is most important. Ca2+ antagonist do not prevent, but partially reverse, endothelin-1-induced contractions indicating that voltage-operated Ca2+ channels do not initiate but contribute to the maintenance of the response.