PHASE-I EVALUATION OF A COMBINATION OF MONOCLONAL-ANTIBODY R24 AND INTERLEUKIN-2 IN PATIENTS WITH METASTATIC MELANOMA
- 1 December 1990
- journal article
- research article
- Vol. 50 (23), 7490-7495
Abstract
A combination of recombinant human interleukin 2 (rhIL-2) and mouse monoclonal antibody R24 (recognizing the ganglioside GD3) was evaluated in patients with metastatic melanoma in a phase I trial. rhIl-2 was given at a constant daily dose of 1 .times. 106 units/m2 i.v. over 6 h on days 1-5 and 8-12. R24 was given on day 8-12 at four dose levels (1, 3, 8, and 12 mg/m2 daily). Twenty patients were evaluable for toxicity and response, five at each dose level. The toxicity of the combination was not overlapping and generally mild. There was a rebound peripheral blood T-lymphocytosis at the end of treatment increasing with the dose of R24. the median lymphocyte count on day 12 of treatment was 3108 .+-. 554/ml in patients treated at R24 doses of 8 and 12 mg/m2 versus 2239 .+-. 672/ml at doses of 1 and 3 mg/m2. This evidence and other data suggested that R24 enhanced IL-2-mediated T-cell activation in vivo. Two patients demonstrated increases in R24-mediated antibody-dependent cellular cytotoxicity for GD3-expressing cells during treatment. rhIL-2 appeared to accelerate the development of human anti-mouse antibody; three patients developed human anti-mouse antibody by the fifth day of R24 treatment, earlier than observed in prior studies using R24 alone and one patient during the first week of rhIL-2 alone, prior to R24 treatment. One patient had a partial response in soft tissue site lasting 6 months and two patients had minor responses. This clinical trial extends the previous observation that R24 enhances lymphocyte proliferation in vitro.This publication has 30 references indexed in Scilit:
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