Determination of Mixtures of Cephacetrile and Ceftezole in a Pure form and Injections, by ‘Zero ‐ Crossing’ Derivative Spectrophotometry

Abstract

A simple and sensitive procedure for the simultaneous determination of cephacetrile and ceftezole in a mixture is presented. First and second derivative spectrophotometry, with a ‘zero - crossing’ technique of measurement, has been used. The methods give linear calibration graphs, with zero intercept on y-axis, for up to 36 μg/ml of cephalosporins. Detection limits of cephacetrile and ceftezole, at p=0.05 level of significance, were calculated to be 0.39 and 0.46 μg/ml in the first derivative mode, and 0.26 and 0.19 μg/ml in the second derivative mode, respectively. The method can be profitably applied to the quantitation of these drugs in injectable dosage forms.