Characteristics of ongoing and reflex discharge of single splenic and renal sympathetic postganglionic fibres in cats.

Abstract

Electrical discharge of thirty-nine single splenic and renal postganglionic nerve fibres was recorded in artificially respired, chloralose-anaesthetized cats. Ongoing discharge rates, averaged over 10 s periods, did not differ between renal and splenic fibres. All neurones of both groups had irregular discharge frequencies. Half of the splenic population and all renal fibres had cardiac-related discharge patterns. Of those tested for respiratory-related firing, 30% of the splenic fibres and 69% of the renal fibres exhibited this pattern. Firing of splenic fibres was less inhibited than that of renal fibres by stimulation of pressoreceptors with phenylephrine-induced increases in blood pressure. Firing of splenic fibres also was less excited than that of renal fibres by unloading pressoreceptors with depressor doses of sodium nitroprusside. Chemical stimulation of splenic afferent nerves with bradykinin consistently elicited greater increases in splenic than renal nerve discharge by causing large increases in firing of all splenic fibres and smaller excitatory responses in 75% of the renal fibres. Application of bradykinin to the intestinal serosa produced greater increases in renal than splenic nerve discharge by consistently causing increased firing of renal fibres and by causing excitation, inhibition, or no change in splenic fibre discharge. Responses of splenic and renal fibres to stimulation of splenic and intestinal afferent nerves after spinal cord transection were similar to those responses elicited when the neuraxis was intact. In conclusion, the differential reflex responses of splenic and renal neuronal populations can be due to the heterogeneity or to the intensity of responses within a neuronal population.