DNA fragments in Friend erythroleukemia cells induced by dimethyl sulfoxide.
- 1 October 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (19), 5912-5915
- https://doi.org/10.1073/pnas.80.19.5912
Abstract
The idea of the correlation between DNA damage caused by dimethyl sulfoxide (Me2SO) and the induction of erythrodifferentiation in murine erythroleukemia (MEL) cells needs reevaluation as a result of the hydroxyapatite chromatographic analysis of DNA from Me2SO-treated and untreated cells as a function of the time in culture. Because Me2SO causes an arrest in G1 phase and consequent delay in DNA synthesis, the valid comparison would be of the DNA samples taken from control and induced cells at the same stage of growth rather than at the same time in culture. At the same stage of growth, there is no evidence of the induction of DNA damage by Me2SO. Presumably, so-called degradation reported in the past stemmed from the fragments of DNA replication.This publication has 17 references indexed in Scilit:
- Indirect induction of erythroid differentiation in mouse Friend cells: evidence for two intracellular reactions involved in the differentiation.Proceedings of the National Academy of Sciences, 1983
- DNA strand breaks and ADP-ribosyl transferase activation during cell differentiationNature, 1982
- An alkaline separation method for detection of small amount of DNA damage.Journal of Radiation Research, 1981
- Amiloride inhibits murine erythroleukemia cell differentiation: evidence for a Ca2+ requirement for commitment.Proceedings of the National Academy of Sciences, 1980
- Measurement of DNA damage in unlabeled mammalian cells analyzed by alkaline elution and a fluorometric DNA assayAnalytical Biochemistry, 1980
- (ADP-ribose)n participates in DNA excision repairNature, 1980
- Induction of murine erythroleukemia differentiation by actinomycin DProceedings of the National Academy of Sciences, 1978
- Erythroleukemic DifferentiationAnnual Review of Biochemistry, 1978
- Effects and possible mechanism of action of dimethylsulfoxide on friend cell differentiationBiochemical Pharmacology, 1978
- Early and late volume changes during erythroid differentiation of cultured friend leukemic cellsJournal of Cellular Physiology, 1977