Features of the pp60v-src carboxyl terminus that are required for transformation.
Open Access
- 1 August 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (8), 2807-2819
- https://doi.org/10.1128/mcb.6.8.2807
Abstract
Analysis of the biological and biochemical activities of pp60recombinant-src proteins encoded by 12 carboxyl-terminal mutants showed that a wide family of alternate src carboxyl termini permit complete transforming and kinase activities. src proteins having carboxyl termini which are up to 10 amino acids longer than that of pp60c-src (17 amino acids longer than that of pp60v-src) still permit transformation. Transformation-positive mutations preserve leucine-516, a residue which is highly conserved in protein-tyrosine kinase sequences; removal causes in vivo protein instability. Successive deletion mutants show that this residue is at the boundary of a region required for kinase activity. pp60src which is truncated just outside this point still transforms cells and binds both pp50 and pp90 cellular proteins.This publication has 68 references indexed in Scilit:
- Expression of a new tyrosine protein kinase is stimulated by retrovirus promoter insertionNature, 1986
- A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRACell, 1985
- Human insulin receptor and its relationship to the tyrosine kinase family of oncogenesNature, 1985
- Expression of v-src and chicken c-src in rat cells demonstrates qualitative differences between pp60v-src and pp60c-srcCell, 1984
- Nucleotide sequences of the drosophila src and abl homologs: conservation and variability in the src family oncogenesCell, 1983
- Revised sequence of the tetracycline-resistance gene of pBR322Gene, 1983
- Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virusCell, 1983
- Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virusNature, 1982
- The specific interaction of the Rous sarcoma virus transforming protein, pp60src, with two cellular proteinsCell, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970