Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex

Abstract

Five new solvent systems are reported for the separation of 1-dimethyl-aminonaphthalene-5-sulphonylamino acids by thin-layer chromatography on silica gel. After two-dimensional chromatography with a suitable pair of these solvent systems, most of the 1-dimethyla-minonaphthalene-5-sulphonyl derivatives were completely separated and could be located by their intense yellow fluorescence when viewed under u.v. light. These techniques have been used to identify 21 amino acids present in superfusates of cat cerebral cortex, plasma and cerebrospinal fluid. A method for the semiquantitative estimation of amino acids in biological fluids is described in which the fluorescent intensity of their separated l-dimethylaminonaphthalene-5-sulphonyl derivatives was measured.