Studies on glycopeptides of herpes simplex virus infected cells

Abstract

Trypsinates from HSV infected cells, radioactively labeled with glucosamine and galactose were further digested by pronase. The digests were subjected to gel filtration, and it was shown that some of the eluted material consisted of saccharides, highly devoid of residual peptide. This material eluted between linear dextran markers of 19,000 and 3000 daltons. Only slight differences between uninfected and HSV infected cells in galactose and glucosamine derived radioactivity profiles of the chromatograms were detected. High molecular weight glucosamine labeled material, present in digest from uninfected cells, was not detectable in digests from HSV infected cells. The concanavalin A adsorbability of eluted fractions was tested. Changes in the relative adsorbability between materials from HSV and uninfected cells were present, especially for the glucosamine labeled saccharides.