Three experiments have been conducted with the aim of producing calves from frozen, sexed embryos by combining embryo splitting and cytogenetic methods. In the first experiment, the efficacy of the bisection technique was assessed by transcervical transfer of 10 monozygotic pairs of half embryos to 10 synchronised heifers. Thirteen calves were produced, including four sets of identical twins. In the second experiment, one of the halves of each of eight split embryos was transferred while the other was processed for sexing by identification of the sex chromosomes. In the third experiment, one of the halves from each of 28 embryos was frozen while the other half was used for sexing. Eleven of the 16 which were sexed have been transferred with the production of three calves of the predicted sex. The overall sexing rate was 60 per cent and the calving rate following transfer of sexed embryos was 60 per cent and 23 per cent for fresh and frozen halves respectively.