Effect of Different Glycerol Levels on the Release of Glutamic Oxaloacetic Transaminase (GOT) from Deep Frozen Ram Spermatozoa

Abstract

Release of GOT was determined during different treatments in ram semen extended (1:10) with lactose-egg yolk diluter containing 3.5% and 5% glycerol (v/v). Semen samples were collected by the electro-ejaculation technique and GOT was analysed colorimetrically. Highly significant difference (p less than 0.01) was found in the post-thaw percent motile spermatozoa and GOT release (IU/10(9) spermatozoa) between 3.5% and 5% glycerol containing equilibrated samples. The difference in percent motile spermatozoa was not significant between 3.5% and 5% glycerol levels in control, post-thaw unequilibrated and prefreeze equilibrated samples. Whereas the difference in GOT release between 3.5% and 5% glycerol concentrations was not significant in control and post-thaw unequilibrated samples and highly significant (p less than 0.01) in prefreeze equilibrated samples.